[发明专利]美洲大蠊断肢再生相关Hedgehog信号通路基因及其dsRNA的应用

摘要

本发明公开了美洲大蠊断肢再生相关基因及其dsRNA的应用。揭示了Hh基因、Ptc基因、Smo基因或/和Ci基因具有影响美洲大蠊断肢再生的能力。而且，沉默Hh、Ptc、Smo和Ci基因的表达，能抑制美洲大蠊断肢再生的能力，即可以高效除去美洲大蠊害虫，又避免了环境污染，安全可靠。本发明Hh、Ptc、Smo和Ci基因可以作为美洲大蠊害虫防治的防治靶点。

主权项

1.抑制Hh、Ptc、Smo或/和Ci表达的试剂在制备阻断美洲大蠊断肢再生的试剂中的应用；所述抑制Hh、Ptc、Smo或/和Ci表达的试剂为抑制Hh、Ptc、Smo或/和Ci RNA转录水平的试剂；所述抑制Hh、Ptc、Smo或/和Ci RNA转录水平的试剂选自沉默Hh、Ptc、Smo或/和Ci表达的siRNA、沉默Hh、Ptc、Smo或/和Ci表达的shRNA和沉默Hh、Ptc、Smo或/和Ci表达的dsRNA的至少一种；所述沉默Hh、Ptc、Smo或/和Ci表达的dsRNA选自以下序列：靶向沉默Hh基因的dsRNA序列：AACACCCUUCCAGCAAGCGGUCUCACCGAGGGGCGCGUGACCCGCAAAGACCCUCGCUUCAGGGAUCUGGUGCCCAACUACAACGCAGACAUCGUGUUCAAGGACGAGGAAGGCACGGGCGCCGACCGCCUCAUGACGCAGAGGUGCAAGGAGAAGCUGAACACGCUGGCCAUCUCGGUGAUGAACCAGUGGCCCGGCGUGAAGCUGCGGGUGACGGAGGGCUGGGACGAGGAGGGGCACCACGCCGUGGACUCGCUGCACUACGAGGGCCGAGCCGUCGACGUCACCACCUCCGACAGGGAUCGAGCCAAGUACGGCAUGCUGGCGCGCCUCGCCGUGGAGGCGGGCUUCGACUGGG(SEQ ID NO.17)；靶向沉默Ptc基因的dsRNA序列：UUCGACGAGCACUAUAUAGACCAAAUAUUCCUAAACAUUAUCCCUUGUGCCAUCAUUACUCCAUUAGACUGUUUUUGGGAAGCUUCGAAGCUUCUGGGACCAGAUUAUCCUGUUCAAAUACCAGGCGUGGGCUCCAAGGUGCGCUGGACGAACCUUAACCCACAACAACUAAUCCAACAAAUGAAGACUGUCGAAUUGGUAUUCCCUUUCGAGACUCUCGAAGACUACAUGAAAAGGGCGGGGAUAAGCACGGGCUACCAAGAGAAGCCGUGUCUCGACCCCUCCGACCCCGAAUGCCCGACCACCGCCCCCAACAAGUUAUCCGGACAGAUACCUGACAUCGGGGCAGAGCUGACAGGAGGCUGCUACGGAUAUGCAGCCAAAUAUAUGCACUGGCCUGAGGACCUGGUGGUGGGCGGAGCCACACGAAAUAAGACUGGACACAUACAAAGGGCGCGUGCAUUGCAGACUGUGGUGCAACUAAUGGGAGAGAGAGAGAUGUAUGAAUUCUGGUCUAAAACGUACAAAGUCCAUCACUUGGAUUGGACACAAGAAAAAGCUGCUUUAAUUCUCGAGACAUGGCAGAGGC(SEQ ID NO.18)；靶向沉默Smo基因的dsRNA序列：ACUUGUAAUUUUCUAUAUCAAUUGCUGUUUUUUGGUGUCAUGUAUUGGAUGGUUGGCACAGUUUAUUCCUGGUGCACGUGAAGGCAUUAUAUGCCGGAAAGAUGGUACUUUAAGGAUGUCAGAACCUAAUACUGGAGAGAAUUUGUCAUGUGUGAUAAUAUUUGUAUUGGUGUAUUACUUCAUCAUGGCUGCCUUUGUGUGGUUUGUAAUUCUCACGUACACAUGGCACAUCAGUUUUCAAGCUCUAGGUAAAAUUCAAGAGCGAAUUGAUAAGAAAAGUGCUUAUUUUCACAUGGUGGCUUGGUCAUUGCCUCUAGUUCUUACUAUUACAACUAUGGCACUAGGGGAAAUAGAUGGCGACAGUGUUGCUGGUAUCUGUUUUGUGGGAUAUGUUAAUCAUGCUGCACGAGCAGGAUUCUUGCUCGUCCCCAUUACAACAGUACUUUUUGUUGGAGGAUACUUUCUCACUAGGGGUCUUAUAACACUGGUGAGACUAAAAAUUAACAGUCAAGAAAUUAUCUCAGAAAGAGCAAGUGCCAAGAUAAGAGAAACCAUUGUGCGCAUGGGUCUCUUUUCACUGUUUACAUUUGUCUUUGUGUU(SEQ ID NO.19)；靶向沉默Ci基因的dsRNA序列：GAUCUCGUCAAGCACAUCAACAAUGACCACAUUCAUGCCAACAAGAAGUCGUUUGUGUGCCGCUGGGAAGACUGUUCAAGGGACGAGAAGCCCUUCAAAGCGCAGUAUAUGCUGGUGGUGCACAUGCGACGACACACAGGGGAGAAGCCCCACAAAUGUACAUUUGAAGGGUGCUACAAAGCCUAUUCGAGGCUGGAGAACCUGAAGACGCACCUCAGAUCUCAUACAGGAGAAAAACCCUACAUGUGUGAGUAUCCUGGAUGCAGCAAAGCCUUCAGCAAUGCGUCCGACCGUGCCAAACACCAGAACCGCACGCACUCCAACGAGAAACCGUACGUGUGUAAGGCCCCGGGCUGCACCAAGAGGUACACAGACCCCAGUUCGCUGCGGAAGCACGUGAAGACAGUGCACGGUGCCGAGUUCUAUGCCAACAAGAAGCACAAGGGAGCUGGCGACGGCGCCGAGGAUGGCUCUGGAGGGGCGGGAGGCCUACUGGACAGCAGCCCGCACAGCGAGGAGAUGAUGAGCGGCAAGACGGCC(SEQ ID NO.20)。