[发明专利]美洲大蠊断肢再生相关Hedgehog信号通路基因及其dsRNA的应用

权利要求书

1.抑制Hh、Ptc、Smo或/和Ci表达的试剂在制备阻断美洲大蠊断肢再生的试剂中的应用；

所述抑制Hh、Ptc、Smo或/和Ci表达的试剂为抑制Hh、Ptc、Smo或/和Ci RNA转录水平的试剂；

所述抑制Hh、Ptc、Smo或/和Ci RNA转录水平的试剂选自沉默Hh、Ptc、Smo或/和Ci表达的siRNA、沉默Hh、Ptc、Smo或/和Ci表达的shRNA和沉默Hh、Ptc、Smo或/和Ci表达的dsRNA的至少一种；

所述沉默Hh、Ptc、Smo或/和Ci表达的dsRNA选自以下序列：

靶向沉默Hh基因的dsRNA序列：

AACACCCUUCCAGCAAGCGGUCUCACCGAGGGGCGCGUGACCCGCAAAGACCCUCGCUUCAGGGAUCUGGUGCCCAACUACAACGCAGACAUCGUGUUCAAGGACGAGGAAGGCACGGGCGCCGACCGCCUCAUGACGCAGAGGUGCAAGGAGAAGCUGAACACGCUGGCCAUCUCGGUGAUGAACCAGUGGCCCGGCGUGAAGCUGCGGGUGACGGAGGGCUGGGACGAGGAGGGGCACCACGCCGUGGACUCGCUGCACUACGAGGGCCGAGCCGUCGACGUCACCACCUCCGACAGGGAUCGAGCCAAGUACGGCAUGCUGGCGCGCCUCGCCGUGGAGGCGGGCUUCGACUGGG(SEQ ID NO.17)；

靶向沉默Ptc基因的dsRNA序列：

UUCGACGAGCACUAUAUAGACCAAAUAUUCCUAAACAUUAUCCCUUGUGCCAUCAUUACUCCAUUAGACUGUUUUUGGGAAGCUUCGAAGCUUCUGGGACCAGAUUAUCCUGUUCAAAUACCAGGCGUGGGCUCCAAGGUGCGCUGGACGAACCUUAACCCACAACAACUAAUCCAACAAAUGAAGACUGUCGAAUUGGUAUUCCCUUUCGAGACUCUCGAAGACUACAUGAAAAGGGCGGGGAUAAGCACGGGCUACCAAGAGAAGCCGUGUCUCGACCCCUCCGACCCCGAAUGCCCGACCACCGCCCCCAACAAGUUAUCCGGACAGAUACCUGACAUCGGGGCAGAGCUGACAGGAGGCUGCUACGGAUAUGCAGCCAAAUAUAUGCACUGGCCUGAGGACCUGGUGGUGGGCGGAGCCACACGAAAUAAGACUGGACACAUACAAAGGGCGCGUGCAUUGCAGACUGUGGUGCAACUAAUGGGAGAGAGAGAGAUGUAUGAAUUCUGGUCUAAAACGUACAAAGUCCAUCACUUGGAUUGGACACAAGAAAAAGCUGCUUUAAUUCUCGAGACAUGGCAGAGGC(SEQ ID NO.18)；

靶向沉默Smo基因的dsRNA序列：

ACUUGUAAUUUUCUAUAUCAAUUGCUGUUUUUUGGUGUCAUGUAUUGGAUGGUUGGCACAGUUUAUUCCUGGUGCACGUGAAGGCAUUAUAUGCCGGAAAGAUGGUACUUUAAGGAUGUCAGAACCUAAUACUGGAGAGAAUUUGUCAUGUGUGAUAAUAUUUGUAUUGGUGUAUUACUUCAUCAUGGCUGCCUUUGUGUGGUUUGUAAUUCUCACGUACACAUGGCACAUCAGUUUUCAAGCUCUAGGUAAAAUUCAAGAGCGAAUUGAUAAGAAAAGUGCUUAUUUUCACAUGGUGGCUUGGUCAUUGCCUCUAGUUCUUACUAUUACAACUAUGGCACUAGGGGAAAUAGAUGGCGACAGUGUUGCUGGUAUCUGUUUUGUGGGAUAUGUUAAUCAUGCUGCACGAGCAGGAUUCUUGCUCGUCCCCAUUACAACAGUACUUUUUGUUGGAGGAUACUUUCUCACUAGGGGUCUUAUAACACUGGUGAGACUAAAAAUUAACAGUCAAGAAAUUAUCUCAGAAAGAGCAAGUGCCAAGAUAAGAGAAACCAUUGUGCGCAUGGGUCUCUUUUCACUGUUUACAUUUGUCUUUGUGUU(SEQID NO.19)；

靶向沉默Ci基因的dsRNA序列：

GAUCUCGUCAAGCACAUCAACAAUGACCACAUUCAUGCCAACAAGAAGUCGUUUGUGUGCCGCUGGGAAGACUGUUCAAGGGACGAGAAGCCCUUCAAAGCGCAGUAUAUGCUGGUGGUGCACAUGCGACGACACACAGGGGAGAAGCCCCACAAAUGUACAUUUGAAGGGUGCUACAAAGCCUAUUCGAGGCUGGAGAACCUGAAGACGCACCUCAGAUCUCAUACAGGAGAAAAACCCUACAUGUGUGAGUAUCCUGGAUGCAGCAAAGCCUUCAGCAAUGCGUCCGACCGUGCCAAACACCAGAACCGCACGCACUCCAACGAGAAACCGUACGUGUGUAAGGCCCCGGGCUGCACCAAGAGGUACACAGACCCCAGUUCGCUGCGGAAGCACGUGAAGACAGUGCACGGUGCCGAGUUCUAUGCCAACAAGAAGCACAAGGGAGCUGGCGACGGCGCCGAGGAUGGCUCUGGAGGGGCGGGAGGCCUACUGGACAGCAGCCCGCACAGCGAGGAGAUGAUGAGCGGCAAGACGGCC(SEQ ID NO.20)。