[发明专利]一种VEGF-A单克隆抗体及试剂盒

权利要求书

1.一种VEGF‐A单克隆抗体，其特征在于，所述抗体的重链可变区氨基酸序列为：MetAla Glu Gly Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr GlnArg Ser Tyr Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln Glu Tyr Pro AspGlu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro Leu Met Arg Cys Gly Gly CysCys Asn Asp Glu Gly Leu Glu Cys Val Pro Thr Glu Glu Ser Asn Ile Thr Met GlnIle Met Arg Ile Lys Pro His Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu GlnHis Asn Lys Cys Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Asn Pro CysGly Pro Cys Ser Glu Arg Arg Lys His Leu Phe Val Gln Asp Pro Gln Thr Cys LysCys Ser Cys Lys Asn Thr Asp Ser Arg Cys Lys Ala Arg Gln Leu Glu Leu Asn GluArg Thr Cys Arg Cys Asp Lys Pro Arg Arg Leu Asn；

轻链可变区氨基酸序列为：Ser Ser Asn Glu His Gly Pro Val Lys Arg Ser SerGln Ser Thr Leu Glu Arg Ser Glu Gln Gln Ile Arg Ala Ala Ser Ser Leu Glu GluLeu Leu Arg Ile Thr His Ser Glu Asp Trp Lys Leu Trp Arg Cys Arg Leu Arg LeuLys Ser Phe Thr Ser Met Asp Ser Arg Ser Ala Ser His Arg Ser Thr Arg Phe AlaAla Thr。

2.一种编码权利要求1所述的轻链可变区和重链可变区氨基酸序列的多核苷酸。

3.一种包含如权利要求2所述的多核苷酸的重组DNA表达载体，所述重组DNA表达载体中包含编码VEGF‐A单克隆抗体重链可变区、轻链可变区和和抗体恒定区的DNA序列。

4.一种包含权利要求1所述VEGF‐A单克隆抗体的检测试剂盒。

5.权利要求4所述检测试剂盒的制备方法，其特征在于，以Nunc化学发光反应板为反应支持物，将VEGF‐A单克隆抗体用0.5mPBS稀释至2.5ug/ml，以100ul/孔包被至化学发光空白板中，4℃下24小时后，用0.9％Nacl洗板3次，加入含有1％BSA的0.5mol PBS封闭，4℃下12小时后甩干。