[发明专利]抗LOX-1人源单链抗体及其制备方法和应用

权利要求书

1.抗LOX-1人源单链抗体，包括轻链和重链，其特征在于，所述抗体包含一组CDR：HCDR1、HCDR2、HCDR3、LCDR1、LCDR2和LCDR3；

HCDR1的氨基酸序列为Gly Asn Ser Ile Ser Arg Gly Ser Tyr Tyr；

HCDR2的氨基酸序列为Val Ser His Ser Gly Ser Thr Tyr；

HCDR3的氨基酸序列为Arg His Gly Leu Gly Ser Arg Ser Gly Tyr Asn Asp AlaPhe；

LCDR1的氨基酸序列为Gln Gly Val Gly Thr Ala；

LCDR2的氨基酸序列为Ala Ala Ser Thr Leu；

LCDR3的氨基酸序列为Gln Gln Tyr Gln Ser Tyr Pro。

2.如权利要求1所述的抗LOX-1人源单链抗体，其特征在于，所述抗体的重链氨基酸序列为Arg Cys Ser Cys Cys Ser Leu Gly Pro Gly Leu Val Lys Pro Ser Glu Thr LeuSer Leu Thr Cys Thr Val Ser Gly Asn Ser Ile Ser Arg Gly Ser Tyr Tyr Trp GlyTrp Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile Gly Ser Val Ser His SerGly Ser Thr Tyr Tyr Asn Pro Ser Leu Lys Ser Arg Val Ile Ile Ser Val Asp ThrCys Lys Asn Gln Leu Ser Leu Lys Leu Asn Ser Val Thr Ala Ala Asp Thr Ala ValTyr Ser Cys Ala Arg His Gly Leu Gly Ser Arg Ser Gly Tyr Asn Asp Ala Phe AspIle Trp Gly Gln Gly Thr Pro Val Thr Val Ser Ser，所述抗体的轻链氨基酸序列为AspIle Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val IleIle Thr Cys Arg Ala Thr Gln Gly Val Gly Thr Ala Leu Ala Trp Phe Gln Gln LysSer Gly Gln Ala Pro Lys Ser Leu Ile Tyr Ala Ala Ser Thr Leu Gln Thr Gly ValPro Ser Thr Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Asn SerLeu Gln Pro Glu Asp Phe Gly Thr Tyr Tyr Cys Gln Gln Tyr Gln Ser Tyr Pro ProThr Phe Gly Gln Gly Thr Arg Leu Glu Ile Lys Arg Ala Ala。

3.如权利要求2所述的抗LOX-1人源单链抗体，其特征在于，所述抗体的轻链可变区和重链可变区通过柔性序列连接，所述柔性序列的氨基酸序列为Gly Gly Gly Gly Ser GlyGly Gly Gly Ser Gly Gly Gly Gly Ser。

4.如权利要求3所述的抗LOX-1人源单链抗体，其特征在于，所述抗体C末端连接6个组氨酸序列，所述组氨酸序列为His His His His His His。

5.如权利要求1-4任一项所述的抗LOX-1人源单链抗体在制备治疗心血管疾病药物中的应用。