[发明专利]一种蛹虫草菌株及其在制备虫草素中的应用

权利要求书

1.蛹虫草菌FY1421（Cordyceps militaris FY1421），其保藏编号为CCTCC NO：M2016220。

2.权利要求1所述蛹虫草菌FY1421在生产虫草素中的应用。

3.一种培养权利要求1所述蛹虫草菌FY1421的方法，包括如下步骤：采用液体发酵培养基培养所述蛹虫草菌FY1421；

所述液体发酵培养基由溶质和溶剂组成；所述溶质及其在所述液体发酵培养基中的浓度如下：葡萄糖2-4g/100ml、蛋白胨1-2g/100ml、酵母粉1-2g/100ml、玉米粉5-6g/100ml、豆饼粉1-3g/100ml、小麦粉0.4-0.6g/100ml、磷酸二氢钾0.4-0.6g/100ml、硫酸镁0.2-0.4g/100ml、维生素B₁ 1-3mg/100ml、维生素B₂ 0.5-1.5mg/100ml、甘氨酸0.2-0.4mg/100ml、苏氨酸0.2-0.3mg/100ml、缬氨酸0.1-0.2mg/100ml、亮氨酸0.04-0.06mg/100ml、异亮氨酸0.05-0.15mg/100ml、苯丙氨酸0.2-0.3mg/100ml、丝氨酸0.05-0.15mg/100ml、脯氨酸0.04-0.06mg/100ml、羟脯氨酸0.02-0.04mg/100ml、半胱氨酸0.01-0.03mg/100ml、色氨酸0.02-0.03mg/100ml、甲硫氨酸0.04-0.06mg/100ml、赖氨酸0.05-0.15mg/100ml、谷氨酸0.04-0.06mg/100ml；所述溶剂为水。

4.一种培养权利要求1所述蛹虫草菌FY1421的方法，依次包括如下步骤（a）和步骤（b）：

步骤（a）：采用种子强化培养基培养蛹虫草菌FY1421；

步骤（b）：将步骤（a）的产物接种至液体发酵培养基进行培养；

所述种子强化培养基的原料配比为：1L营养液：0.5-1.5kg小米；所述营养液由溶质和溶剂组成；所述溶质及其在所述营养液中的浓度如下：马铃薯浸出粉1-3g/100ml、葡萄糖0.5-1.5g/100ml、蛋白胨0.4-0.8g/100ml、磷酸二氢钾0.05-0.15g/100ml、硫酸镁0.04-0.06g/100ml、维生素B₁ 1-3mg/100ml、维生素B₂ 0.5-1.5mg/100ml、甘氨酸0.2-0.4mg/100ml、苏氨酸0.2-0.3mg/100ml、缬氨酸0.1-0.2mg/100ml、亮氨酸0.04-0.06mg/100ml、异亮氨酸0.05-0.15mg/100ml、苯丙氨酸0.2-0.3mg/100ml、丝氨酸0.05-0.15mg/100ml、脯氨酸0.04-0.06mg/100ml、羟脯氨酸0.02-0.04mg/100ml、半胱氨酸0.01-0.03mg/100ml、色氨酸0.02-0.03mg/100ml、甲硫氨酸0.04-0.06mg/100ml、赖氨酸0.05-0.15mg/100ml、谷氨酸0.04-0.06mg/100ml；所述溶剂为水；

所述液体发酵培养基由溶质和溶剂组成；所述溶质及其在所述液体发酵培养基中的浓度如下：葡萄糖2-4g/100ml、蛋白胨1-2g/100ml、酵母粉1-2g/100ml、玉米粉5-6g/100ml、豆饼粉1-3g/100ml、小麦粉0.4-0.6g/100ml、磷酸二氢钾0.4-0.6g/100ml、硫酸镁0.2-0.4g/100ml、维生素B₁ 1-3mg/100ml、维生素B₂ 0.5-1.5mg/100ml、甘氨酸0.2-0.4mg/100ml、苏氨酸0.2-0.3mg/100ml、缬氨酸0.1-0.2mg/100ml、亮氨酸0.04-0.06mg/100ml、异亮氨酸0.05-0.15mg/100ml、苯丙氨酸0.2-0.3mg/100ml、丝氨酸0.05-0.15mg/100ml、脯氨酸0.04-0.06mg/100ml、羟脯氨酸0.02-0.04mg/100ml、半胱氨酸0.01-0.03mg/100ml、色氨酸0.02-0.03mg/100ml、甲硫氨酸0.04-0.06mg/100ml、赖氨酸0.05-0.15mg/100ml、谷氨酸0.04-0.06mg/100ml；所述溶剂为水。