[发明专利]特异性双链RNA结合蛋白嵌合体及其在病毒感染性疾病中的应用无效
| 申请号： | 200910021226.9 | 申请日： | 2009-02-20 |
| 公开（公告）号： | CN101525388A | 公开（公告）日： | 2009-09-09 |
| 发明（设计）人： | 费舟;杨静华;樊代明;汪爱勤;程光;晁晓东 | 申请（专利权）人： | 中国人民解放军第四军医大学 |
| 主分类号： | C07K19/00 | 分类号： | C07K19/00;C12N15/63;A61K38/17;A61P31/12 |
| 代理公司： | 西安慈源有限责任专利事务所 | 代理人： | 鲍燕平 |
| 地址： | 710032*** | 国省代码： | 陕西;61 |
| 权利要求书： | 查看更多 | 说明书： | 查看更多 |
| 摘要： | |||
| 搜索关键词： | 特异性 rna 结合 蛋白 嵌合体 及其 病毒感染 性疾病 中的 应用 | ||
1.特异性双链RNA结合蛋白嵌合体，其特征在于将蛋白转导信号，双链RNA感受器 和细胞凋亡信号通过DNA重组的方法按以上顺序连接起来，组成特异性双链RNA结合蛋 白嵌合体，并分别在原核和真核细胞中表达；所述的特异性双链RNA结合蛋白嵌合体，其 cDNA和氨基酸序列如下：
No.1 dsCARE PKR
cDNA序列：
ATGTACGCCCGTGCCGCCGCCCGTCAGGCCCGTGCCAGTGGTCTCGAGATGGCTG
GTGATCTTTCAGCAGGTTTCTTCATGGAGGAACTTAATACATACCGTCAGAAGCAG
GGAGTAGTACTTAAATATCAAGAACTGCCTAATTCAGGACCTCCACATGATAGGAG
GTTTACATTTCAAGTTATAATAGATGGAAGAGAATTTCCAGAAGGTGAAGGTAGAT
CAAAGAAGGAAGCAAAAAATGCCGCAGCCAAATTAGCTGTTGAGATACTTAATAA
GGAAAAGAAGGCAGTTAGTCCTTTATTATTGACAACAACGAATTCTTCAGAAGGAT
TATCCATGGGGAATTACATAGGCCTTATCAATAGAATTGCCCAGAAGAAAAGACTA
ACTGTAAATTATGAACAGTGTGCATCGGGGGTGCATGGGCCAGAAGGATTTCATTA
TAAATGCAAAATGGGACAGAAAGAATATAGTATTGGTACAGGTTCTACTAAACAGG
AAGCAAAACAATTGGCCGCTAAACTTGCATATCTTCAGATATTATCAGAAGAAACC
TCAGTGAAATCTGACTACCTGTCCTCTGGTTCCGGTACCATGGATGCAAAAGCTCG
AAATTGTTTGCTTCAACATAGAGAAGCTCTGGAAAAGGACATCAAGACATCCTACA
TCATGGATCACATGATTAGTGATGGATTTTTAACAATATCAGAAGAGGAAAAAGTA
AGAAATGAGCCCACTCAACAGCAAAGAGCAGCTATGCTGATTAAAATGATACTTAA
AAAAGATAATGATTCCTACGTATCATTCTACAATGCTCTACTACATGAAGGATATAA
AGATCTTGCTGCCCTTCTCCATGATGGCATTCCTGTTGTCTAA
蛋白序列：
MYARAAARQARASGLEMAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFT
FQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGL
INRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYL
QILSEETSVKSDYLSSGSGTMDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLTISEEEK
VRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAALLHDGIPVV
No2.dsCARE ADAR1
cDNA序列：
ATGTACGCCCGTGCCGCCGCCCGTCAGGCCCGTGCCAGTGGTCTCGAGAAGAACCCCA
TCAGCGGGCTGTTAGAATATGCCCAGTTCGCTAGTCAAACCTGTGAGTTCAACATGATA
GAGCAGAGTGGACCACCCCATGAACCTCGATTTAAATTCCAGGTTGTCATCAATGGCC
GAGAGTTTCCCCCAGCTGAAGCTGGAAGCAAGAAAGTGGCCAAGCAGGATGCAGCTA
TGAAAGCCATGACAATTCTGCTAGAGGAAGCCAAAGCCAAGGACAGTGGAAAATCAG
AAGAATCATCCCACTATTCCACAGAGAAAGAATCAGAGAAGACTGCAGAGTCCCAGA
CCCCCACCCCTTCAGCCACATCCTTCTTTTCTGGGAAGAGCCCCGTCACCACACTGCTT
GAGTGTATGCACAAATTGGGGAACTCCTGCGAATTCCGTCTCCTGTCCAAAGAAGGCC
CTGCCCATGAACCCAAGTTCCAATACTGTGTTGCAGTGGGAGCCCAAACTTTCCCCAG
TGTGAGTGCTCCCAGCAAGAAAGTGGCAAAGCAGATGGCCGCAGAGGAAGCCATGAA
GGCCCTGCATGGGGAGGCGACCAACTCCATGGCTTCTGATAACCAGCCTGAAGGTATG
ATCTCAGAGTCACTTGATAACTTGGAATCCATGATGCCCAACAAGGTCAGGAAGATTG
GCGAGCTCGTGAGATACCTGAACACCAACCCTGTGGGTGGCCTTTTGGAGTACGCCCG
CTCCCATGGCTTTGCTGCTGAATTCAAGTTGGTCGACCAGTCCGGACCTCCTCACGAG
CCCAAGTTCGTTTACCAAGCAAAAGTTGGGGGTCGCTGGTTCCCAGCCGTCTGCGCAC
ACAGCAAGAAGCAAGGCAAGCAGGAAGCAGCAGATGCGGCTCTCCGTGTCTTGATTG
GGGAGAACGGTACCATGGATGCAAAAGCTCGAAATTGTTTGCTTCAACATAGAGAAGC
TCTGGAAAAGGACATCAAGACATCCTACATCATGGATCACATGATTAGTGATGGATTTTT
AACAATATCAGAAGAGGAAAAAGTAAGAAATGAGCCCACTCAACAGCAAAGAGCAGC
TATGCTGATTAAAATGATACTTAAAAAAGATAATGATTCCTACGTATCATTCTACAATGCT
CTACTACATGAAGGATATAAAGATCTTGCTGCCCTTCTCCATGATGGCATTCCTGTTGTC
TAA
蛋白序列：
MYARAAARQARASGLEKNPISGLLEYAQFASQTCEFNMIEQSGPPHEPRFKFQVVINGREF
PPAEAGSKKVAKQDAAMKAMTILLEEAKAKDSGKSEESSHYSTEKESEKTAESQTPTPSAT
SFFSGKSPVTTLLECMHKLGNSCEFRLLSKEGPAHEPKFQYCVAVGAQTFPSVSAPSKKVA
KQMAAEEAMKALHGEATNSMASDNQPEGMISESLDNLESMMPNKVRKIGELVRYLNTN
PVGGLLEYARSHGEAAEFKLVDQSGPPHEPKFVYQAKVGGRWFPAVCAHSKKQGKQEAA
DAALRVLIGENGTMDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLTISEEEKVRNEPT
QQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAALLHDGIPVV
No3.dsCARE PACT
cDNA序列：
ATGTACGCCCGTGCCGCCGCCCGTCAGGCCCGTGCCAGTGGTCTCGAGAGCGATTACG
ACATCCCCACTACTGAGAATCTTTATTTTCAGGGATCCTCCCAGAGCAGGCACCGCGCC
GAGGCCCCGCCGCTGGAGCGCGAGGACAGTGGGACCTTCAGTTTGGGGAAGATGATA
ACAGCTAAGCCAGGGAAAACACCGATTCAGGTATTACACGAATACGGCATGAAGACCA
AGAACATCCCAGTTTATGAATGTGAAAGATCTGATGTGCAAATACACGTGCCCACTTTC
ACCTTCAGAGTAACCGTTGGTGACATAACCTGCACAGGTGAAGGTACAAGTAAGAAG
CTGGCGAAACATAGAGCTGCAGAGGCTGCCATAAACATTTTGAAAGCCAATGCAAGTA
TTTGCTTTGCAGTTCCTGACCCCTTAATGCCTGACCCTTCCAAGCAACCAAAGAACCA
GCTTAATCCTATTGGTTCATTACAGGAATTGGCTATTCATCATGGCTGGAGACTTCCTGA
ATATACCCTTTCCCAGGAGGGAGGACCTGCTCATAAGAGAGAATATACTACAATTTGCA
GGCTAGAGTCATTTATGGAAACTGGAAAGGGGGCATCAAAAAAGCAAGCCAAAAGGA
ATGCTGCTGAGAAATTTCTTGCCAAATTTAGTAATATTTCTCCAGAGAACCACATTTCTT
TAACAAATGTAGTAGGACATTCTTTAGGATGTACTTGGCATTCCTTGAGGAATTCTCCTG
GTGAAAAGATCAACTTACTGAAAAGAAGCCTCCTCAGTATTCCAAATACAGATTACATC
CAGCTGCTTAGTGAAATTGCCAAGGAACAAGGTTTTAATATAACATATTTGGATATAGAT
GAACTGAGCGCCAATGGACAATATCAATGTCTTGCTGAACTGTCCACCAGCCCCATCA
CAGTCTGTCATGGCTCCGGTATCTCCTGTGGCAATGCACAAAGTGATGCAGCTCACAAT
GCTTTGCAGTATTTAAAGATAATAGCAGAAAGAAAGGGTACCATGGATGCAAAAGCTC
GAAATTGTTTGCTTCAACATAGAGAAGCTCTGGAAAAGGACATCAAGACATCCTACAT
CATGGATCACATGATTAGTGATGGATTTTTAACAATATCAGAAGAGGAAAAAGTAAGAA
ATGAGCCCACTCAACAGCAAAGAGCAGCTATGCTGATTAAAATGATACTTAAAAAAGA
TAATGATTCCTACGTATCATTCTACAATGCTCTACTACATGAAGGATATAAAGATCTTGCT
GCCCTTCTCCATGATGGCATTCCTGTTGTCTAA
蛋白序列：
MYARAAARQARASGLESDYDIPTTENLYFQGSSQSRHREAPPLEREDSGTFSLGKMITAK
PGKTPIQVLHEYGMKTKNIPVYECERSDVQIHVPTFTFRVTVGDITCTGEGTSKKLAKHRA
AEAAINILKANASICFAVPDPLMPDPSKQPKNQLNPIGSLQELAIHHGWRLPEYTLSQEGGP
AHKREYTTICRLESFMETGKGASKKQAKRNAAEKFLAKFSNISPENHISLTNVVGHSLGCT
WHSLRNSPGEKINLLKRSLLSIPNTDYIQLLSEIAKEQGFNITYLDIDELSANGQYQCLAEL
STSPITVCHGSGISCGNAQSDAAHNALQYLKIIAERKGTMDAKARNCLLQHREALEKDIK
TSYIMDHMISDGFLTISEEEKVRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYK
DLAALLHDGIPVV
No4.dsCARE NF90
cDNA序列：
ATGTACGCCCGTGCCGCCGCCCGTCAGGCCCGTGCCAGTGGTCTCGAGATGAATGCCC
TGATGCGGTTGAACCAGCTGAAGCCAGGGCTGCAGTACAAGCTGGTGTCCCAGACTG
GGCCCGTCCATGCCCCCATCTTTACCATGTCTGTGGAGGTTGATGGCAATTCATTCGAG
GCCTCTGGGCCCTCCAAAAAGACGGCCAAGCTGCACGTGGCCGTTAAGGTGTTACAG
GACATGGGCTTGCCGACGGGTGCTGAAGGCAGGGACTCGAGCAAGGGGGAGGACTC
GGCTGAGGAGACCGAGGCGAAGCCAGCAGTGGTGGCCCCTGCCCCAGTGGTAGAAGC
TGTCTCCACCCCTAGTGCGGCCTTTCCCTCAGATGCCACTGCCGAGAACGTAAAACAG
CAGGGGCCGATCCTGACAAAGCACGGCAAGAACCCAGTCATGGAGCTGAACGAGAA
GAGGCGTGGGCTCAAGTACGAGCTCATCTCCGAGACCGGGGGCAGCCACGACAAGCG
CTTCGTCATGGAGGTCGAAGTGGATGGACAGAAGTTCCAAGGTGCTGGTTCCAACAA
AAAGGTGGGTACCATGGATGCAAAAGCTCGAAATTGTITGCTTCAACATAGAGAAGCT
CTGGAAAAGGACATCAAGACATCCTACATCATGGATCACATGATTAGTGATGGATTTTT
AACAATATCAGAAGAGGAAAAAGTAAGAAATGAGCCCACTCAACAGCAAAGAGCAGC
TATGCTGATTAAAATGATACTTAAAAAAGATAATGATTCCTACGTATCATTCTACAATGCT
CTACTACATGAAGGATATAAAGATCTTGCTGCCCTTCTCCATGATGGCATTCCTGTTGTC
TAA
蛋白序列：
MYARAAARQARASGLEMNALMRLNQLKPGLQYKLVSQTGPVHAPIFTMSVEVDGNSFE
ASGPSKKTAKLHVAVKVLQDMGLPTGAEGRDSSKGEDSAEETEAKPAVVAPAPVVEAVST
PSAAFPSDATAENVKQQGPILTKHGKNPVMELNEKRRGLKYELISETGGSHDKRFVMEVE
VDGQKFQGAGSNKKVGTMDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLTISEEEK
VRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAALLHDGIPVV
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