[发明专利]草鱼肠道细胞胞质苹果酸脱氢酶基因序列无效
| 申请号: | 200810147928.7 | 申请日: | 2008-12-19 |
| 公开(公告)号: | CN101434961A | 公开(公告)日: | 2009-05-20 |
| 发明(设计)人: | 徐恒;朱文漓;辜文博;顾继锐;吴江;王娟 | 申请(专利权)人: | 通威股份有限公司;四川大学 |
| 主分类号: | C12N15/53 | 分类号: | C12N15/53;C12N9/04;C12N15/10 |
| 代理公司: | 暂无信息 | 代理人: | 暂无信息 |
| 地址: | 610041四*** | 国省代码: | 四川;51 |
| 权利要求书: | 查看更多 | 说明书: | 查看更多 |
| 摘要: | |||
| 搜索关键词: | 草鱼 肠道 细胞 苹果酸 脱氢酶 基因 序列 | ||
1、一种草鱼草鱼肠道细胞胞质苹果酸脱氢酶基因,其特征在于具有以下RNA核苷酸及相应的氨基酸序列:
gctgagtggc agagcagcac atctttggcg cacatctcaa cctgcacaat cagacttgaa 60
ac atg gcc gaa ccg atc cgt gtc ctg gtg act 92
Met Ala Glu Pro Ile Arg Val Leu Val Thr 10
1 5 10
ggc gca gcc gga cag atc gcc tat tct ctg 122
Gly Ala Ala Gly Gln Ile Ala Tyr Ser Leu 20
11 15 20
ctc tac agc att gct aaa gga gat gtg ttc 152
Leu Tyr Ser Ile Ala Lys Gly Asp Val Phe 30
21 25 30
ggc aag gac cag cca atc atc ttg gtg ctt 182
Gly Lys Asp Gln Pro Ile Ile Leu Val Leu 40
31 35 40
ctg gac atc act ccc atg ctg cct gtg ctg 212
Leu Asp Ile Thr Pro Met Leu Pro Val Leu 50
41 45 50
gat ggg gtc gtc atg gaa ctg cag gat tgt 242
Asp Gly Val Val Met Glu Leu Gln Asp Cys 60
51 55 60
gct ctt cct ctt ctg agg gat gtg att cct 272
Ala Leu Pro Leu Leu Arg Asp Val Ile Pro 70
61 65 70
act gat aag gtt gag gtg ggc ttc aag gac 302
Thr Ala Lys Val Glu Val Gly Phe Lys Asp 80
71 75 80
ctt gat gct gcc atc ttg gtg ggc tct atg 332
Leu Asp Ala Ala Ile Leu Val Gly Ser Met 90
81 85 90
cca agg aaa gag ggc atg gag aga aag gac 362
Pro Arg Lys Glu Gly Met Glu Arg Lys Asp 100
91 95 100
ctt ctg aag gcc aac gtg gcc att ttt aaa 392
Leu Leu Lys Ala Asn Val Ala Ile Phe Lys 110
101 105 110
acc caa ggt gaa gca ctg gag aag tac gcc 422
Thr Gln Gly Glu Ala Leu Glu Lys Tyr Ala 120
111 115 120
cag aag act gtc aag gtg cta gtt gtc ggg 452
Gln Lys Thr Val Lys Val Leu Val Val Gly 130
121 125 130
aat cca gcc aat acc aac tgt ttg atc gcc 482
Asn Pro Ala Asn Thr Asn Cys Leu Ile Ala 140
131 135 140
tcc aaa tct gct ccg tcc att cct aag gag 512
Ser Lys Ser Ala Pro Ser Ile Pro Lys Glu 150
141 145 150
aac ttc tcc tgc ctg acc cgt ctg gac cat 542
Asn Phe Ser Cys Leu Thr Arg Leu Asp His 160
151 155 160
aac agg gcc cgc tct cag gtg gcg atg cgt 572
Asn Arg Ala Arg Ser Gln Val Ala Met Arg 170
161 165 170
gtt ggt gtg tcc tct gac aat gtg aag aat 602
Val Gly Val Ser Ser Asp Asn Val Lys Asn 180
171 175 180
gtg att atc tgg gga aat cac tcc tca act 632
Val Ile Ile Trp Gly Asn His Ser Ser Thr 190
181 185 190
cag tac cca gat gtg cac cac gct atc gtg 662
Gln Tyr Pro Asp Val His His Ala Ile Val 200
191 195 200
aac cac cat ggg aag gag ttg gca gcc ttt 692
Asn His His Gly Lys Glu Leu Ala Ala Phe 210
201 205 210
gac gcc gtg aat gat gaa agc tgg ctg aag 722
Asp Ala Val Asn Asp Glu Ser Trp Leu Lys 220
211 215 220
ggc gac ttc atc tcc acg gtg cag cag aga 752
Gly Asp Phe Ile Ser Thr Val Gln Gln Arg 230
221 225 230
ggt gca gct gtc atc aag gcc agg aag ctc 782
Gly Ala Ala Val Ile Lys Ala Arg Lys Leu 240
231 235 240
tcc agc gca atg tct gct gcc aaa gcc atc 812
Ser Ser Ala Met Ser Ala Ala Lys Ala Ile 250
241 245 250
tgt gac cac atg agg gac atc tgg ttc ggc 842
Cys Asp His Met Arg Asp Ile Trp Phe Gly 260
251 255 260
act cct gat ggt gag tgg gtg tct atg ggc 872
Thr Pro Asp Gly Glu Trp Val Ser Met Gly 270
261 265 270
gtc tac tcc tct ggt aat tcc tat gga gtt 902
Val Tyr Ser Ser Gly Asn Ser Tyr Gly Val 280
271 275 280
cct gat gat ctc atg tac tcc ttc cct gtt 932
Pro Asp Asp Leu Met Tyr Ser Phe Pro Val 290
281 285 290
aag att aag aac aag acc tgg aag gtg gtt 962
Lys Ile Lys Asn Lys Thr Trp Lys Val Val 300
291 295 300
gac gga ctc tcc atc aac gat ttc tcc cgc 992
Asp Gly Leu Ser Ile Asn Asp Phe Ser Arg 310
301 305 310
gct aag atg gac gcc acc tcc gct gag ctg 1022
Ala Lys Met Asp Ala Thr Ser Ala Glu Leu 320
311 315 320
gta gag gag aga gac acg gca gtc acc ttc 1052
Val Glu Glu Arg Asp Thr Ala Val Thr Phe 330
321 325 330
ctt gga gcg 1061
Leu Gly Ala 333
331 333
tgactcgact ccacgacaac accgcagcag ttagaccctt caaagggccc aaatccccga 1121
ctgtagaatt cacagctgac cgtgtgtgtg tgtgtgtgtg tgtgtggata ttcatgtatc 1181
ttaacgtatg cgtgtgtgcg tgtgtaactt ctcaagaaac gtttgcaatg taacttactc 1241
tgcttggagt gtccggggca acacctgaag gagaaccttt ccaattataa ccgttacgca 1301
catggttgtc ctgaccaaaa tgatgatcat ctgtcataca acaataaaag tacatgggaa 1361
aaaaaaaaaa aaaaaaaaaa aaaaactcga 1391
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