[发明专利]皂苷的新用途及其分离方法

说明书

本发明涉及在其糖残基之一上包含乙酰基残基的皂苷的新用途。这些皂苷能够比已知的皂苷，甚至是Lipofectamin增强转染效率至令人惊讶地高得多的程度。

描述

本发明涉及根据权利要求1的前序部分的皂苷的体外用途，根据权利要求3的前序部分的此类皂苷的体内用途，根据权利要求7的前序部分的转染组合物，根据权利要求10的前序部分的体外转染方法，以及根据权利要求15的前序部分的分离此类皂苷的方法。

过去已经描述了许多皂苷。示例性地，参考Weng,Alexander等人A simplemethod for isolation of Gypsophila saponins for the combined application oftargeted toxins and saponins in tumor therapy.Planta medica 75(13)(2009):1421-1422；Weng,Alexander等人A convenient method for saponin isolation intumour therapy.Journal of Chromatography B 878(7)(2010):713-718；Weng,Alexander等人The toxin component of targeted anti-tumor toxins determinestheir efficacy increase by saponins.Molecular oncology 6(3)(2012):323-332；和Weng,Alexander等人Saponins modulate the intracellular trafficking of proteintoxins.Journal of controlled release 164(1)(2012):74–86。

其它皂苷描述于以下出版物中：Thakur、Mayank等人High-speedcountercurrent chromatographic recovery and off-line electrospray ionizationmass spectrometry profiling of bisdesmodic saponins from Saponariaofficinalis possessing synergistic toxicity enhancing properties on targetedantitumor toxins.Journal of Chromatography B 955(2014):1-9；Jia,Zhonghua,Kazuo Koike和Tamotsu Nikaido.Major triterpenoid saponins from Saponariaofficinalis.Journal of natural products 61(11)(1998):1368-1373；Haddad,Mohamed等人New triterpene saponins from Acanthophyllum pachystegium.Helvetica chimica acta 87(1)(2004):73-81；Fu,Hongzheng等人“SilenorubicosidesA-D,Triterpenoid Saponins from Silene rubicunda.”Journal of Natural Products68(5)(2005):754-758；Moniuszko-Szajwaj,Barbara等人Highly Polar TriterpenoidSaponins from the Roots of Saponaria officinalis L.Helvetica Chimica Acta 99(5)(2016):347-354；Fuchs,Hendrik等人Glycosylated Triterpenoids as EndosomalEscape Enhancers in Targeted Tumor Therapies.Biomedicines 5(2)(2017):14。