[发明专利]一种石斛兰的组培快繁培养基

权利要求书

1.一种石斛兰组培快繁培养基，包括诱导培养基、继代培养基、壮苗培养基，其特征在于各培养基的具体组成如下：

诱导培养基：WPM培养基+玉米素0.5mg/L-0.8mg/L+NAA0.2mg/L-0.3mg/L+琼脂5.0g/L-8.0g/L+蔗糖2.0g/L-4.0g/L+黄秋葵汁5.0g/L-8.0g/L+辣木叶汁12.0g/L-15.0g/L，pH值5.2-5.3；

继代培养基：WPM培养基+活性炭1g/L+赤霉素0.5mg/L-0.8mg/L+萘乙酸2mg/L-3mg/L+琼脂5.0g/L-8.0g/L+黄秋葵汁5.0g/L-8.0g/L+辣木叶汁12.0g/L-15.0g/L+蔗糖2.0g/L-4.0g/L，pH值5.5-5.6；

壮苗培养基：1/2MS+活性炭1g/L+吲哚丁酸0.4mg/L-0.6mg/L+萘乙酸0.5mg/L-0.6mg/L+琼脂5.0g/L-8.0g/L+黄秋葵汁5.0g/L-8.0g/L+辣木叶汁12.0g/L-15.0g/L+蔗糖2.0g/L-4.0g/L，pH值5.5-5.6。

2.根据权利要求1所述的石斛兰组培快繁培养基，其特征在于：所述诱导培养基的组成为：WPM培养基+玉米素0.6mg/L+NAA0.25mg/L+琼脂6.0g/L+蔗糖3.0g/L+黄秋葵汁7.0g/L+辣木叶汁14.0g/L，pH值5.2。

3.根据权利要求1所述的石斛兰组培快繁培养基，其特征在于：所述继代培养基的组成为：WPM培养基+活性炭1g/L+赤霉素0.6mg/L+萘乙酸2.5mg/L+琼脂6.0g/L+黄秋葵汁7.0g/L+辣木叶汁14.0g/L+蔗糖3.0g/L，pH值5.5。

4.根据权利要求1所述的石斛兰组培快繁培养基，其特征在于：所述壮苗培养基的组成为：1/2MS+活性炭1g/L+吲哚丁酸0.5mg/L+萘乙酸0.5mg/L+琼脂6.0g/L+黄秋葵汁7.0g/L+辣木叶汁14.0g/L+蔗糖3.0g/L，pH值5.6。

5.根据权利要求1所述的石斛兰组培快繁培养基，其特征在于各培养基的具体组成如下：

诱导培养基：WPM培养基+玉米素0.6mg/L+NAA0.25mg/L+琼脂6.0g/L+蔗糖3.0g/L+黄秋葵汁7.0g/L+辣木叶汁14.0g/L，pH值5.2；

继代培养基：WPM培养基+活性炭1g/L+赤霉素0.6mg/L+萘乙酸2.5mg/L+琼脂6.0g/L+黄秋葵汁7.0g/L+辣木叶汁14.0g/L+蔗糖3.0g/L，pH值5.5；

壮苗培养基：1/2MS+活性炭1g/L+吲哚丁酸0.5mg/L+萘乙酸0.5mg/L+琼脂6.0g/L+黄秋葵汁7.0g/L+辣木叶汁14.0g/L+蔗糖3.0g/L，pH值5.6。

6.根据权利要求1-5中任一项所述的石斛兰组培快繁培养基，其特征在于：所述辣木叶汁的制备方法是：先洗净原料，然后充分捣碎，再用纱布过滤，所述滤液即为辣木叶汁。

7.根据权利要求1-5中任一项所述的石斛兰组培快繁培养基，其特征在于：所述黄秋葵汁是将黄秋葵表皮洗净后，用机器搅打成汁得到的。