[发明专利]运用比较基因组学快速鉴定荠菜抗白粉病基因

权利要求书

1.荠菜抗白粉病基因，其特征在于选自下列4个基因或其之一：

carubv10022893m.g

氨基酸序列：

MAIKERTLEETPTWAVAVVCFVLLFISIMIEYFLHFVGHWFKKKHKKALYEALEKVKAELMLLGFISLLLVVLQDPVSQICIPRKVASTWHPCTHHQEMVKYGKDYVDDGRKILEDFDSTDVFGPRRSLATKGYDKCAEKGKVALVSAYGIHQLHIFIFVLAVFHILYCIITYALGKTKMKKWKSWEKETKTIEYQYANDPERFRFARDTSFGRRHLNMWSKSSVTLWITCFFRQFFGSVTKVDYLTLRHGFIMAHLPAGSAARFDFQKYIQRSLEEDFTVVVGISPLIWCIAVLFILTNTNGWASYLWLPFIPLLVILVVGAKLQMIISKLGLRIQDKGDVVKGAPVVEPGDDLFWFGRPRLILFLIHLVLFTNAFQLAFFVWSTYEFTLRNCFHHKNEDIAIRITMGVLIQVICSYITLPLYALVTQMGTSMRPTIFNDRVANALKKWHHTAKKQTKHGQSGSNTPHSSRPATPTHGMSPVHLLHNYHNRSSLDQQTSFTASPSPPRFSDYSGHGHGHHQFFDPESQNHSYQREITDSEFSNSHHPHTDVASPVREEMQIVEHVKVDLPEFTFKK

核苷酸序列：

ATGGCGATAAAAGAGCGAACACTGGAGGAGACACCAACATGGGCTGTTGCTGTTGTTTGCTTCGTTCTCCTTTTCATTTCCATCATGATCGAATATTTCTTGCACTTTGTTGGTCACTGGTTTAAAAAGAAGCACAAGAAAGCTTTATATGAAGCTCTTGAAAAGGTTAAAGCAGAATTGATGCTACTGGGATTCATATCGCTTCTATTGGTGGTATTGCAAGATCCAGTCTCTCAGATTTGCATTCCAAGAAAAGTTGCTTCGACTTGGCATCCTTGTACCCACCATCAAGAAATGGTTAAATACGGTAAAGATTATGTCGACGATGGCCGCAAGATTCTTGAAGACTTTGACTCCACCGACGTTTTTGGTCCTCGCCGCAGTTTAGCCACCAAAGGTTATGACAAATGCGCAGAAAAGGGAAAAGTAGCATTAGTATCTGCATATGGTATCCACCAGCTGCATATATTCATCTTTGTGCTTGCTGTTTTTCATATCCTCTACTGCATTATAACCTATGCTTTGGGGAAGACCAAGATGAAGAAATGGAAGTCATGGGAGAAAGAGACCAAAACAATTGAGTACCAATATGCCAATGATCCCGAGAGGTTCAGATTTGCAAGAGACACATCGTTCGGACGTAGACATTTGAACATGTGGAGCAAGTCTTCCGTTACCCTCTGGATTACATGTTTCTTCAGACAATTCTTTGGATCAGTGACAAAAGTGGATTATCTTACACTAAGACATGGCTTTATTATGGCGCATTTGCCAGCAGGAAGTGCAGCTCGTTTCGATTTCCAAAAATACATTCAAAGATCTTTGGAAGAAGATTTCACGGTGGTGGTCGGCATAAGTCCATTGATATGGTGCATTGCTGTCTTATTCATATTGACCAACACAAATGGATGGGCTTCATATCTTTGGTTGCCCTTCATCCCATTGCTTGTGATATTGGTAGTAGGAGCAAAACTTCAAATGATAATATCCAAGTTAGGATTAAGGATTCAAGACAAAGGAGATGTGGTTAAAGGAGCTCCTGTGGTTGAACCGGGTGATGATCTCTTCTGGTTTGGTCGTCCTCGTTTGATCCTCTTCCTCATCCACTTGGTCCTTTTCACGAATGCATTTCAACTGGCTTTCTTCGTTTGGAGCACTTACGAATTCACACTCCGAAACTGCTTCCACCACAAAAACGAAGATATTGCAATTAGGATCACCATGGGGGTATTAATACAAGTTATATGCAGCTACATCACTCTACCTCTCTATGCTCTTGTTACTCAGATGGGAACTTCAATGAGGCCGACCATATTCAACGATAGGGTAGCAAATGCATTGAAGAAATGGCACCACACAGCCAAGAAACAGACGAAACATGGACAGTCGGGATCTAACACACCTCACTCTAGCCGCCCTGCTACGCCAACTCATGGCATGTCACCGGTGCATCTCCTCCACAACTACCATAACCGCAGCAGCCTCGACCAACAAACCAGCTTCACGGCCTCTCCTTCTCCTCCTAGATTCTCTGATTATAGCGGCCATGGCCATGGCCATCATCAGTTCTTCGACCCTGAATCTCAGAATCACTCTTACCAGCGTGAGATCACAGATTCTGAATTCAGCAACAGTCATCATCCCCATACTGACGTTGCAAGTCCTGTTAGAGAAGAGATGCAGATTGTTGAGCATGTCAAGGTTGATTTGCCTGAATTTACCTTCAAGAAGTAG

carubv10009086m.g

氨基酸序列：

MADATLEETSTWTVAVVCFVLLLISIALEHLLHTLGTWFKKKHKSDLFEALEKVKAELMLLGFISLLLTIAQAPISHICISKKLASIMQPCSPAQAAKLKADRAKKEGGYGEKPGGKLLLELAEFYIPRRSLAGKVENTCEKKGKVPFVSYYGIHQLHIFIFVLAVVHIIYCIVIYALGKTKMRRWQPWEEEAKSIEYQFSNDPERFRFARDTSFGRRHLSFWSKRSVTLWTVCFFRQFFGSITKVDYLTLRHGFITAHFAPGQSSYDFRKLIQRTLEKDFKTVVEISPVIWFVAVLFLLTNTSGLYSHFWLPFIPLVVSLIVGTKLEVIITQLGQSIQEKDEIVRGAPVVRPKDDLFWFSKPRFVLFLIHLVLFTNAFQLAFFAWSTFSFGIKNCFHASIPDVVTRLVSFCTDTLQLCDSSTLCTCYSHGYSNEANSIQRRSSHSIREVASDGEEPD

核苷酸序列：

ATGGCAGACGCGACTTTAGAGGAGACCTCTACGTGGACAGTAGCTGTGGTTTGCTTCGTCTTACTACTCATTTCGATTGCGCTCGAACATTTGCTTCACACACTTGGAACCTGGTTTAAAAAGAAGCACAAAAGCGATCTATTTGAAGCTCTTGAAAAGGTCAAAGCAGAGCTTATGTTGTTGGGATTCATATCACTACTCCTAACAATTGCACAAGCACCAATCTCACATATTTGCATCTCCAAGAAACTTGCATCGATAATGCAACCTTGCAGTCCCGCCCAAGCAGCGAAATTAAAGGCCGATAGGGCAAAGAAAGAAGGTGGATATGGAGAAAAACCCGGTGGAAAACTTCTTCTTGAGCTGGCTGAATTTTATATCCCTAGAAGAAGTTTAGCCGGCAAAGTTGAGAACACATGCGAAAAGAAGGGGAAAGTGCCTTTTGTATCTTATTATGGAATCCACCAGCTGCATATATTCATCTTCGTGCTCGCAGTGGTTCATATTATTTACTGCATTGTTATTTATGCTCTCGGAAAGACCAAGATGAGAAGGTGGCAGCCGTGGGAGGAGGAGGCAAAGTCAATAGAGTATCAGTTTTCCAACGATCCTGAGAGGTTCAGGTTTGCGAGGGACACATCTTTTGGGAGAAGACATCTCAGTTTCTGGAGCAAGAGGAGTGTTACACTATGGACTGTGTGTTTTTTCAGACAGTTCTTTGGATCCATCACCAAAGTTGATTACTTAACATTAAGACATGGTTTTATCACGGCGCATTTCGCTCCCGGCCAATCAAGCTACGATTTCCGTAAGTTAATCCAGAGAACGTTAGAGAAAGACTTCAAAACCGTTGTTGAAATCAGTCCTGTTATCTGGTTTGTCGCCGTGCTATTCCTCTTGACCAATACAAGTGGGTTATATTCTCACTTCTGGTTACCATTCATTCCACTAGTCGTAAGTCTAATCGTTGGAACAAAGCTTGAAGTCATAATAACACAATTGGGTCAAAGTATCCAAGAGAAAGACGAAATAGTGAGAGGCGCCCCTGTGGTTCGGCCTAAAGATGACCTCTTCTGGTTTAGCAAGCCACGTTTCGTTCTTTTCCTTATCCACTTGGTCCTCTTTACGAATGCATTTCAACTTGCCTTCTTTGCCTGGAGCACGTTTTCATTCGGGATCAAGAATTGTTTCCATGCAAGCATTCCAGATGTGGTCACTAGACTTGTTAGCTTTTGTACAGATACTTTGCAGCTATGTGACTCTTCCACTCTATGCACTTGTTACTCACATGGGTACTCAAATGAAGCCAACAGTATTCAACGAAGGAGTAGCCACAGCATTAGAGAAGTGGCATCAGACGGCGAAGAACCAGACTAA

carubv10008691m.g

氨基酸序列：

MANQVKERTLEETSTWAVAVVCFVLLFISIVLEHSIHKIGTWFRKKHKKALFEALEKVKAELMLLGFISLLLTIGQTPISNICISEQVASTMHPCSAAEEARKYHKKDPAKKEHGDGDGEKSGRRLLLELAESYIPRRSLATKGYDKCAEKGKVAFVSAYGIHQLHIFIFVLAVVHVIYCIVTYALGKTKMRRWKSWEEETKTIEYQYSNDPERFRFARDTSFGRRHLNFWSKSSVTLWVVCFFRQFFGSVTKVDYLALRHGFIMAHLAHGSESRFDFRKYIQRSLEEDFKTVVEISPVIWF VAVLFLLTNTNGLRSYLWLPFIPLIVILIVGTKLQVIITKLGLRIQEKGDVVRGAPVVQPGDDLFWFGKPRFILFLIHLVLFTNAFQLAFFAWSTYEFNLRNCFHESNADVVIRIAVGVVVQILCSYVTLPLYALVTQMGTKMKPTVFNERVATALKKWHHTAKSQTKHGRHSESNTPFSSRPTTPTHGSSPIHLLHNFNNRNVDSYPTSPSPRYSGHGHHDEHQFWDPESQRQEAETSTHHSLAHESSERKPALASVELPPIRTSKSLKDFSFKR

核苷酸序列：

ATGGCGAATCAAGTTAAGGAGCGGACTTTAGAAGAGACCTCTACGTGGGCAGTAGCTGTGGTTTGCTTCGTCTTACTATTTATTTCGATTGTTCTCGAACATTCGATTCACAAAATTGGAACCTGGTTTAGAAAGAAGCACAAGAAGGCTCTATTTGAAGCTCTTGAAAAGGTCAAAGCAGAGCTTATGCTGTTGGGATTCATATCACTACTCCTCACTATTGGACAAACGCCAATCTCAAATATCTGCATCTCCGAGCAGGTTGCATCGACAATGCACCCTTGCAGCGCCGCGGAAGAAGCTAGAAAATACCACAAGAAAGACCCCGCAAAGAAAGAACATGGAGATGGAGATGGAGAAAAATCGGGTCGAAGACTTCTTCTTGAGTTAGCTGAATCTTATATCCCTAGACGAAGTTTAGCCACCAAAGGTTATGACAAATGTGCAGAGAAGGGGAAAGTGGCTTTTGTATCTGCTTATGGAATCCACCAGTTGCATATATTCATCTTTGTGCTGGCCGTGGTTCATGTTATTTATTGCATTGTTACTTATGCTCTCGGAAAGACTAAGATGAGGAGGTGGAAGTCATGGGAGGAGGAGACGAAGACAATAGAGTATCAGTATTCCAATGATCCTGAGAGGTTCAGGTTTGCGAGGGACACATCCTTTGGGAGAAGACATCTCAATTTCTGGAGTAAGTCGAGTGTTACACTATGGGTTGTGTGTTTTTTCAGACAGTTCTTTGGATCTGTCACCAAAGTTGATTACTTAGCACTAAGACATGGTTTCATCATGGCGCATTTGGCTCACGGGAGCGAATCAAGATTTGATTTCCGCAAGTATATTCAGAGATCATTAGAGGAAGACTTCAAAACTGTTGTTGAAATCAGTCCGGTTATTTGGTTTGTCGCGGTGCTATTCCTCTTGACCAATACAAATGGACTACGTTCTTACCTTTGGTTACCATTTATTCCACTAATCGTAATTCTAATAGTTGGAACAAAGCTTCAAGTCATAATTACCAAATTGGGTCTAAGAATCCAAGAGAAAGGTGATGTGGTGAGAGGTGCTCCGGTGGTTCAGCCTGGTGATGACCTCTTCTGGTTTGGCAAGCCACGGTTCATTCTTTTTCTTATCCACTTGGTCCTCTTTACGAATGCATTTCAACTAGCCTTCTTTGCCTGGAGTACGTATGAGTTCAATCTCAGGAATTGTTTCCATGAAAGCAATGCAGATGTGGTCATTAGGATTGCAGTTGGAGTTGTTGTGCAGATACTTTGCAGCTATGTGACTCTTCCACTCTATGCACTTGTTACTCAGATGGGTACTAAGATGAAGCCAACAGTATTTAACGAAAGAGTAGCCACAGCATTAAAGAAGTGGCATCACACGGCAAAGAGCCAGACTAAACACGGAAGACACTCTGAATCAAACACACCTTTCTCTAGCCGTCCAACCACACCAACACATGGCTCATCTCCCATCCATCTCCTTCACAATTTCAATAACCGGAACGTTGACAGTTACCCAACTTCTCCTTCTCCTAGATACTCTGGTCATGGTCATCATGATGAACACCAGTTTTGGGATCCTGAGTCTCAACGCCAAGAAGCTGAAACGTCCACACATCATTCTCTTGCGCATGAAAGCTCAGAAAGGAAGCCGGCTCTTGCATCTGTTGAACTTCCTCCAATAAGGACTAGCAAAAGCTTAAAAGATTTCTCTTTTAAGAGATGA

carubv10020030m.g

氨基酸序列：

MAEQVKEKTLEETSTWAVAVVCFVLLLISIVIENLIHKIGSWFKNKNKKALYEALEKVKAELMLMGFISLLLTIGQGYISNICIPKNIAASMHPCSAAEEARKYGKKLVPKVQEDGGDDDDNSRRKLLQLVDSIIPRRSLATKGYDKCAEKGKVAFVSAYGMHQLHIFIFVLAVCHVIYCIVTYALGKTKMRRWKRWEQETKTIEYQYSHDPERFRFARDTSFGRRHLNFWSRTTVTLWIVCFFRQFFRSVTKVDYLTLRHGFIMAHLAPGSDARFDFRKYIQRSLEEDFKTVVEINPVIWFVAVLFLLTNTNGLNSYLWLPFIPFVVILIVGTKLQVIITKLGLRIQEKGDVVKGTPLVQPSDHFFWFGRPRFILFLIHLVLFTNAFQLAFFVWSTYEFGLKNCFHESSVDVIIRISIGLIVQILCSYVTLPLYALVTQMGSKMKPTVFNERVAIALKSWHHTAKKQIKHGRTSESTTPFSSRPTTPTHGSSPIHLLRNVPHKRSRSVDDNSFANSMSPRRNSDFDSWDPESQHETAETSTNSNHRSRFREEESEKKLGPSSSVELPPGPGQIRTQHDISNISLRDFSFKR

核苷酸序列：

ATGGCGGAACAAGTGAAAGAAAAAACATTAGAGGAAACTTCTACATGGGCCGTCGCTGTGGTTTGCTTCGTCTTGCTTCTGATTTCTATTGTTATTGAGAATCTTATTCATAAAATTGGATCCTGGTTTAAAAACAAGAACAAAAAAGCTTTATATGAAGCTCTTGAAAAAGTGAAAGCAGAGCTTATGCTGATGGGATTCATATCACTACTGCTTACTATTGGACAAGGCTACATCTCGAATATTTGCATCCCTAAGAACATCGCAGCGTCGATGCACCCTTGTAGTGCAGCCGAAGAAGCGAGAAAGTACGGTAAGAAATTAGTCCCAAAGGTCCAGGAAGACGGAGGAGACGACGATGACAACTCGCGTCGAAAGCTTCTCCAATTAGTCGATTCTATCATTCCTCGAAGGAGTTTGGCTACCAAAGGTTATGATAAGTGTGCAGAGAAGGGGAAAGTCGCTTTTGTATCGGCTTATGGCATGCATCAGCTGCATATATTCATCTTTGTTCTTGCGGTTTGTCATGTGATTTACTGCATTGTTACTTATGCTTTGGGGAAGACCAAGATGAGAAGGTGGAAGAGGTGGGAACAGGAGACGAAGACAATCGAATATCAGTATTCACACGATCCCGAGAGGTTTAGGTTCGCGAGGGATACATCTTTCGGGCGTAGACATCTGAATTTCTGGAGCAGAACAACTGTTACGCTGTGGATTGTATGTTTCTTCAGACAGTTCTTTAGATCAGTAACCAAAGTTGATTACTTAACACTGAGACATGGTTTCATCATGGCTCATTTGGCTCCTGGGAGCGATGCAAGATTCGATTTCCGAAAGTACATTCAGAGATCATTAGAGGAAGACTTTAAAACCGTTGTCGAGATCAATCCTGTGATCTGGTTCGTCGCTGTCTTGTTTCTCCTGACCAACACAAACGGATTGAATTCTTACCTCTGGTTACCTTTCATTCCTTTTGTCGTGATTCTTATCGTTGGGACAAAACTTCAAGTGATAATAACAAAACTAGGACTACGAATCCAAGAGAAAGGCGACGTAGTAAAAGGCACACCGCTTGTTCAGCCCAGTGATCATTTCTTCTGGTTCGGTCGCCCACGTTTCATTCTCTTCCTCATTCACTTGGTTCTCTTCACGAACGCGTTTCAACTCGCTTTCTTTGTCTGGAGTACGTATGAATTCGGTCTAAAGAACTGTTTCCATGAAAGCAGTGTAGATGTAATCATCAGAATCTCAATCGGACTTATTGTCCAGATTCTTTGCAGCTACGTTACTCTTCCTCTTTACGCTCTTAGTCACTCAGATGGGTTCAAAGATGAAACCAACAGTGTTCAACGAGAGAGTAGCAATAGCATTAAAGAGTTGGCACCACACAGCAAAGAAACAGATCAAACATGGAAGAACCTCAGAATCAACCACACCTTTCTCTAGCCGACCAACAACACCAACACATGGTTCTTCTCCGATTCATCTCCTCCGCAACGTCCCTCACAAACGAAGCAGAAGCGTTGACGATAACAGCTTTGCGAATTCAATGTCTCCGAGAAGAAACTCTGATTTCGATTCGTGGGATCCTGAGTCTCAACACGAAACTGCTGAGACTTCGACGAACTCAAATCATCGTTCAAGGTTTAGAGAAGAAGAATCGGAGAAAAAGCTTGGACCTTCTTCATCTGTTGAACTTCCTCCTGGACCTGGTCAGATACGAACTCAGCATGATATTAGTAATATCAGCTTAAGGGATTTTTCGTTTAAGCGGTGA。

2.权利要求1所述快速鉴定荠菜白粉病基因的应用，包括：

1)携带有抗白粉病MLO基因的育种材料的创制或者新品种创制：利用权利1所给出的MLO基因源为亲本材料，在杂交、回交后代中，可以获得转育有MLO抗性基因的育种材料。

2)抗白粉病基础理论研究：对权利1的MLO型抗白粉病基因进行分子标记分析；对该基因进行分子作图或者基因定位；对该基因进行克隆以及基因间互作分析。

3)抗白粉病转基因研究：利用权利要求1所给出的MLO型白粉病基因进行转基因研究。