[发明专利]一种草菇V23菌株的特异分子标记及其获得方法与应用

权利要求书

1.一种草菇V23菌株的特异分子标记，其PCR扩增引物对序列为：GTGACTGCTATGGAACACATTGGAC和TCGGAGGAAGCGGGTCCACTACA。

2.根据权利要求1所述的一种草菇V23菌株的特异分子标记，其特征在于：所述的特异分子标记为分子量为4430bp和6224bp的两条条带；其中长度6224bp的序列如下：

GTGACTGCTATGGAACACATTGGACGATGGCGGATCAATGAAGAGAGTAATATCTTAGATAGCCATTAACAATGTCTAGATAGAAATTTGTTGGGTTCTC TCTCCTCATGCGAAGTGAAAGCAGGAGTCGTATAGAACTAGGGCTACTTCAGTAATGCATTATCCTGCAAAAGTTCCAGATTTACAATCCCGACAACCCG CTGTTGTGGATGACAATCGGCTTAGAAAAGGTCCCAAAGACACGAGCAAGAGAAACCAAAACCATCCAGGGAGCCTCGCCATACGAAGAGCAACGGGAGG TCGCAATGAAATCATTACCAAGGAGTACCTGTGATACTGTAGGACCCAAGAGGTGAGATTGTACAAATTTTGTACATCAGCCGCCCAAAGTGTGCATTAG CCAAGACGTTAAATTAACTTGTGATGGTTGTCTAGATAAGATAATATGAGTAGTACTCATTTCTTTCTTATGATTCTTTACCACGTCTCATCCAGTGAAT GATTTTGCACAGCCCCACTTCATAAGGTGACCTGTGCTTCGGTATAAGAGAGTTGCATAACAATCCAGACAAAAGTGTTGTATCTGAGGACAATGGTGTT AAAAAGACCCTTAAATCGTGTGTTGATCACCCACACCATCCCACTCTCCGACTTGCCCCACCAGCATAGATATCCTTAGTTTACTCTAGGATAAACCTGG GCAGCCTGCTGCCTACATCTTGGCACTTGCTTGCCTGCAGCGGTTAATAAATGGGATTTCAATAACCACCTTAAATCATGTGACCATCCATGCCCTGAGT ATAGACTACTATAAAATTACGGTTTCATTCATCTTGATTACAAAAACGCGCGCGCTTGGTGCTTCCGAGTACATTAACTATGAATCCGTAAACGTTTTGA TTTTATTCATACAAATTGTGTGGAAGCAACATGGGAATCTAAACCCACAATGCCTCAGTGCGACACGAACATGATTGTTGTTAGTTTACAAATAGATAGC GAGAGCTGAGATGTGTGGTGACGATTGGGTATCAGTTGAAGTGAAGGGTATACTTAGTATACATTATGAGGGCGTTGGGTGTAGGAATGCATGGTCTCCA AGGAGCTATAACTGGCAAGGTTAGTGTCATAAATTCAGCTGGGAAGAGCGCTGTCAGGGGTgATACTCGACGTGAGCAACACAGATAGTGTCGGGTCATA AGCATTTAtATCACGAAGGTCAGAGGGAGTGGAAATATCGGCGAAATCAAATAGCAATGCTAACCAAGAATGCCATCAGGTTGTGAATAAGAGTGAATTA GATGGCACTGACTAGGTTGTACACCCGCAGATTGCTCGCCCATGATCCAACTCCAATCTTCAATCGGTGAAGACAGGAAATCGAAACCGTGGATGGGTGC CAGAAGGGGGTTTTGCTGTGAGATACTAGAACTGTCAAAATCATGATCTATTTCGACAATCAGTACACAGCTAAATGCACGGGAAAATGACGTACCCTTT GTGATAAACCCAGTCGAAATATCGGAAAATGAACCCAGATGCATGGTTGAGTAATCAAACAATTCGACGTCGAGGTTCGTGGTGGGTTCATGCAGATCTG TAACAATGGACTGGGTGTAGGCTTGTTGAAACCAATCATCCAGATGCTTCACATCGTATGTCATGGAATTGGATAGAGGTTCTGATACAACATAAGTAGG CGAATCACTGTCATTACCTCGAAGCCGCTTAAGGGAGATTACTGTTGCTCCTTCAGACAGACGGCGTTTTCGGCTACCCGAAGGCATAGGCAGTGGGGAT GACATAGATAGGGGCGTATCTGGCGTGGGAGGAAAAGGTTGCCTAGGCAAGTCAGGAAGGGCAGCTAGGAGCTCCGGGTAAGACGATGGAGATGAGGGCG AAGTCGGTGTGCCTAATTGTAGTGGCATCTGAGTGCGTCTAGTATTGACGTCCGGTGTGACAGCGTCAGGACTACAATCGACAGAGTGAGGTGGCACAGG AGAGCGACAAGGGGAGGGAGAGGGAGAGGGATAACATGAAGTGACATCGTGGCTAGGAGATTGCATAGCATGATGTGGCTCACTGACGATCTGGGATACA AGACCTGATTCGAATAGTCTCTTGGAGTATAGGGCCAGTGTGGCTTGACGGACCTTCGTGAACGCGGACGCTACTCTAGAATCCACGCATTGCGATTTTG ATTTCGTCGAGAAGAATAGGCGTGCGGCGTCGACAATGGCATTGCGTTTGTTCGAAAAATGAGTTTTGCGCAGGGCATTCCAACCTATTCGCTTTCTAAC ATCGATGAACCAGTTGTCGATATCCTTTCTGGAACATCCGGTTGATCGCGATATGGATTCCCTCACAGCTGAAGAGGGATATGGATTATGCAGATTATGC AGCAGCCAATCATACGCTGGTTGGATATACCTGGCCACAGTATCTGCAGGCGAATGGTGAACCATGTAGACAAGGCCTAAATATTtGTAACACCCACCTG GAAATGATGGTGATTCTCGGGGAGCGTCTTGAACTAGAGTTTCATCAATTTCTTTCATAAGTTGATGTTCGATGGCAGTTGCTGTGTCTTCAATCTCCAG

长度4430bp的序列如下：

AAGGAGGCTATGAAACACATTGGACGGTGGCGAATAGAGGAGGAAGGTCATGTCTTAAGAGGCCACTAAAATCATACATATATTTGAGCATACTCGTTACATCCTCGCCATAAATATATAATGTATTGCGAGACCATTTAAATTTTTTATACCACTGAGGTACCTTCAGAAATCGGTGCACAGCCTGAGCAATGGCTTTGGAGATCCTCCCTTGTTGACCCTGATTGAAGGATATCCCCCCTCCAAGACGAAAACCTGCATACTTTCCAAGATGTACGGGGCCCGTACTTGAAACAAATTGGGGGTGAGCAAGTCGATAGAAAGCCCGCAGTGTCCCAGGAATTGTGCTCATGGAGCGAGTTCGTCGGTGTCGAAGCATTACCTGTTAAATCCCCGCGCTACTACAGGCAGAGAGACGGGTGACATGTCCAAAGAATACGAGCCAGTGCTGATCGCCTCCTACGATATTAGGGGATCCGAGTGTGTAGGTATTAACGCGCTCGGTAGCTCCTTTTCGAGAGGTGCCAAAAGAATCGTGTCATCTAACCAGTCGAGTGGAGGTAGAGGGTTCGTGTAAGGTAGTGCAGGATAAGATAAACTATTGCCACCAACGGCAAGGTTGTCGACCAAGTCACTGTTTAATCCAGAATTGGAGACTTGAAAGGCGGAATTGAGATCAGAGTGTATGAGTGGTCCTAGTATACGTATAGTGTTAGCGAACATGAAGCGAAGCGAATTGATGTGAATATATACCTTGCTCATCGCCGCCGTTCAACTCATTGAATGCGTGAATAGTGCTGGAGCCTCGTATTTCTAATGGATTCGAGACCTCGGGAGGATCAAATAAGTCTTGGGAAGATGAAAAAGGAAGCTCAATCAAGGCGGTGGATGGATCCAGTGACTGAGAATCGAATAGAGAGACATCAAGTGGAACAGACCCGTTGCTGGACCAAATGGTCTCAAATTCATTGCTAAACCAGGATTCTAAAGTTTCTATGTCGATGGACGCATGAAGGGGCGAATTAGAGTTGTCATATTGATCCCGTGGTCGTTTAGGGCGGCGTTGATCAGCATCTGAGAGACATCGCTTGCGTCTGAGAGGGGAAGGCCTTGAAGGTAATATTGTGGATTGCCCTGGATCATTATACCTGAAGACAAGCCCTCCAGTAAGCAAAAATGGGACATGTACTGAAACCCTGGACTTACTCGAAGGGCCGATTGACAGAGCCCTCACCATCTGATCCTGGGGCAGCTATTGCAGGTGTATTCTTAGGAAGAGGTGATAACGGTGAGTAGTGGCAATTCGGCAAGCTGGCAGTCTGTAGATTAGGTGGGGTTAAAAGTGGAGGCGTAGAACAAGCTGATGAATTAGACTTTAGAGTGTCACGGTCCGGAGTAGGATGATCTAGAGTTGCTGCTAGGATAGTTTCTTTAAAACGACCTGAGTAAAGTTCCATAGCTTTGCTCCGGATCTTTTCGAAAGCAGCGAGAAGCTTTGGATCAACAAGGCTGTAATCTGCATTGGAAATGAAGGCACGGGTCGCAGCTTCCACTATCTCCTTGCGTTTGTTATGAAAGCGCGACTTCCGCAAAGTGTTCCATCCAATTCGCCGGCGAGCATCGACAAACCAGGAATCGATGTTTTGTCGCGGAGTTCCACTCGCATTTGCAATTGTGTCCTTCTGACTCGAGGATGGATAAGGGTTGTGAAGGTTGCGCAAGAGCCAGTCACAGGCCAATGGTATGTAGCTAGGTGCTTGAAGGCTAAACGTCCGATCTTCGGTCTCGGGATATTCTTCAGCAAGTATTGACTCTGTATCCCGGACAAGGCGCTCGGTAATTTCAGTGTAGGTCTCTTCCAAGTCCAAGTAAGATGTGATTATGGTACACATCGACTGAGATATTGTGTAGGCGAGATGATTTAGATCGTCGTCAATGGTGCCACGACTGGAGCAGTCATCATACAAAGAACATGTGTGTGTCCATGTTTCGTGCAATTTTGCGACAGTATCCTCGTCACCTCGTTCCAGGACATCGAAAAGCAGCCGCTGGCTTTCAAGGAGGTAATGGCGTAGGTCGGTTGTAAATACCCGCGTAGACATCATATGGTGGTTGGGATGGAAGGTTGTGAAGTGTTGTTTGTAGCTAACACCACAGCTTAAGTAATTGCCCTTCTGGGTGAACAATGGCATCCTCGTTGAACTCGTGCATCATACCCATCCAGCCTCACCACACTGACGAACATACGGTCGAGCCCCAACATCATGTCCCGTTCCGCCCTTGAAGGTATCATGGCTGTGGCCAATCGCACACAGCAGATGTTACCACCTTCGACCGCCTTGCGCAAGCCTATCCCACCACAAACCCTCACAGCACGGCCAGCATTAGATTTACACCTTCCCAAGCACATCCAAGCCGACATTGATTGCCTACCCCCCGATGCCAGACAAGTTATATCTCGCCAGATTGACGCCCTCAACTCGTTGTATCACTCTTCATACGCGAAATCCTGTGCTGGAGTCGAAGCTAATCCATTGCCTCAGTTACCAGATGCTCGCACTTTTCAGCGCCTTCGCTCATGCTTCGAAAATATGTATCGACAGAAAGAGATGCCTAGAGTTGAAGCGTGGATAGAAAGCGCAAGGGCGAAGCGGCGGAGAGTTTCAAAGGACGAGAATTCGACACAACGACCACGGTTTAAACATGTAAGGACTTGCCTCACTACATCAGCGTGATCACCATTGATGAAATATTACAGGAGTATTCTCAGCTACTGGAAAATTACTTCTCCAAGAACGCATACCCATCACGTCCAGATCGAGCAGTGCTTGCGAGGAAATCTGGCATGACACTGAAGCAAATTGAGGTGTGGGTGAGTATTTATTCTATGTCGTGTAATCGTTACACCCACTCGCTCACCCGTTTTTTTTTCTTTCTCAGTTTCAGAATCATAGAAGCAGAGCCAAAAAGGAAGGTCGAGCACTGAGTCGACCCAAGAACCCAGAATCCCCCAGTATTGTACGGCCTTCCGCTAGTTATGAAGGTGATCGTGTCACTGGAATAAGTGCGGCGACAACGACAATTGTTGAGGTTTGTAGTGTTTCTGGCGTTTGGTTATATACTCACGCCTCGCTACAGAGGTGTAATCTGCTAAGTGATTCTGACCCCTCACCGGGCACCTTTCCATGCAGATATAGTTTACAACAACCTGGCATAAAACCTACGCTCGTTCCGATTGTCTGGCCTCGAAGTGCATCCATATGCACTTATAACAACAGGACAGACGTTGACATAGACAGCCTCTGCACAAATTTTGCACTGAAGTTGCAATTTAGAGACCGTAGGGAAAAGTCAAGCCGTTCTTCAACGCCTGTCAGGCAATCTCATTTGTCTTCCTTCTATACTTACGCAGTCCCTGCTCCTTTACCTGCCCTCAGACAGGAGATTCAAACTGTTGATGAAGCTCTGCGACCCCTGCGACCAAAGGAAGCAACGTTCGGTTCCCGTGGGGGCATTCTTACACGATCTCCTATTCGTTCAATCTCCAGACACGCACCCTTCGCTCCATATATTATAGCTAATACGCAACCATCATTCAGATCTCATACCCAACGTATACTCCGTGTCCTCCCCCCTCCCAAACCGTCAATGGTCAACGACATTTATCACAGCGCTCCCGTAGCACCATTAAGCCTCGATTCAATTGAATACTGGCCACCACTTGCACCTTCATGCTCGTCGCAACTGACCCAAATTCCGTCATCGACTCAACCCCTATCCTTCGGAATAGTGGTGTAACCTCTCAAAACTCCACTCTCATGCAAGCCTTATTTATGACTAATTCATGTGCCCTAGGGGACGTAATGCCAATGTAAAGGTGTACACCGATTGGAAAACTTGGATGCCTTCAAGGGAATTAGGATTCTGACTAGTATCATGTTGTGAATTTGTCGATACTAGAGCCGCCATGCAGGGATGGCACCGAGTTGTGCAGCATGACCCACTTGCCTGCTACCATTGCCAATCGCGGGTACACGGGCAGTGACGCCCACGGAATCAGAGAACTACCACTGTCCGGTAATAATATGCAATATATGGCCTATCGAGTCGGCTGTCAAGAAAGCAAAGCCACAGCAACAAGGTTGCAGGCGGTTCAGCCAGTCGGACAAAGTAGCTACGTTGCGTACATGCAATCACGGAGTGGATGCTCGTGTAGATAATGTATTTAGGCCGATTTGAATAAATTGAACAAATTGCGCAAATCCTTGTAAGTGACCAATTCTGACTTGCAAATGCATCACTTCCTGATTTGAGATGCCTAAGGCAAATTAGCAGGGAAGAATGATAAAGGCTTTGCTCACGCACTAAATTTAACAAACCACCAAGGCCCCCAGAATTTGAGCCCCCTGTAGTGGTCCCACTTCCTCCAA。