[发明专利]一种稳定性更好、灵敏度更高的钾离子试剂及检测方法

摘要

本发明涉及血清钾离子检测技术领域，特别涉及一种钾离子检测试剂，试剂R1中含有咪唑缓冲液，MIT,BSA,蔗糖，海藻糖，GLDH，LDH，PK，聚乙二醇，MgCl₂，十二烷基三甲基氯化铵，月桂基咪唑啉甜菜碱，防腐剂；试剂R2中含有Tris缓冲液，PEP_·BSA，蔗糖_·海藻糖，EDTA，NADH，防腐剂。采用咪唑缓冲液，添加稳定剂BSA,蔗糖，海藻糖，聚乙二醇，大大增强了试剂的稳定性；采用新型表面活性剂十二烷基三甲基氯化铵，月桂基咪唑啉甜菜碱，不仅显著改善测定的性能，而且增强了试剂的稳定性和分析灵敏度。

主权项

一种钾离子检测试剂，其特征在于包括试剂R1和试剂R2，所述试剂R1和试剂R2的组成如下：试剂R1中含有钾离子的检测试剂，包试剂R1和试剂R2：1）其R1的组成为：咪唑‑HCL缓冲液_{········································}100mmol/L，MIT_{···················································}3g/L，BSA_{···················································}1g/L，ɑ‑KG_{··················································}5g/L，MgCl_{2·················································}15mmol/L，ADP_{···················································}5mmol/L，蔗糖_{··················································}5g/L，海藻糖_{················································}2g/L，聚乙二醇_{··············································}5g/L，黄原胶_{················································}2g/L，山梨醇_{················································}4g/L，甘油_{··················································}120g/L，麦芽糖_{················································}5g/L，GLDH_{··················································}5KU/L，LDH_{···················································}5KU/L，PK_{····················································}3KU/L，曲拉通X‑100·_{·········································}2mL/L，十二烷基三甲基氯化铵_{··································}0.5g/L，月桂基咪唑啉甜菜碱 _{···································}3ml/L，防腐剂_{················································}0.5g/L；2）试剂R2的组分为：Tris‑HCL缓冲液_{········································}100mmol/L，PEP_{···················································}2KU/L，BSA_{···················································}1g/L，NADH_{··················································}3mmol/L蔗糖_{··················································}5g/L，海藻糖_{················································}2g/L，EDTA_{··················································}2g/L，防腐剂_{················································}0.5g/L。