[发明专利]一种稳定的同型半胱氨酸检测试剂

摘要

本发明提供了一种稳定的同型半胱氨酸检测试剂，包括试剂1和试剂2，本发明对试剂1和试剂2进行了改进，使本发明与同类检测试剂对比相关性好，临床检测样本结果一致，能够达到市场对产品的应用要求，并且灵敏度高，抗干扰性能好，是一种更加稳定、良好的同型半胱氨酸检测试剂。

主权项

一种稳定的同型半胱氨酸检测试剂，其特征在于，包括体积比为240:65的试剂1和试剂2，试剂1和试剂2的组成及含量如下：试剂1溶剂                             PIPES（哌嗪‑1,4‑二乙磺酸）缓冲液，pH=8.0，25℃ S‑腺苷甲硫氨酸                                                              0.15 mmol/LNADH                                                                                   0.35 mmol/L三（2羧乙基）膦氯化氢                                                 0.6 mmol/L                α－酮戊二酸                                                                     5.3 mmol/L乙二胺四乙酸二钠                                                                1mmol/L椰油酰胺丙基甜菜碱                                                            3g/L叠氮钠                                                                              0.5g/L ；试剂2溶剂                                           HEPES缓冲液，pH=7.0，25℃，Hcy甲基转移酶                                                           1.5KU/L谷氨酸脱氢酶                                                        1.8KU/L              S‑腺苷同型半胱氨酸水解酶                                   3.0KU/L腺苷脱氨酶                                                                    5.5KU/L聚乙二醇6000                                                                  1g/L牛血清白蛋白                                                                1g/L椰油酰胺丙基甜菜碱                                                        3g/L叠氮钠                                                                             0.5g/L  。