[发明专利]用于检测EGFR基因拷贝数和7号染色体倍性的试剂

摘要

本发明公开了一种用于检测EGFR基因拷贝数和7号染色体倍性的试剂，所述试剂包括用于检测EGFR基因拷贝数的荧光探针RP11-339F13和用于检测7号染色体倍性的荧光探针RP11-144H20；其中，RP11-339F13为覆盖EGFR基因全长的BAC克隆，用罗丹明荧光素标记；RP11-144H20为7号染色体着丝粒特异的BAC克隆，用异硫氰酸荧光素标记。使用本发明试剂可以快速、简便地检测细胞内EGFR基因拷贝数及7号染色体倍性，有助于药物敏感性和预后的判断，对指导于非小细胞肺癌等肿瘤分子靶向药物酪氨酸激酶抑制剂(TKIs)的使用具有重要意义。

主权项

1.用于检测EGFR基因拷贝数和7号染色体倍性的试剂，其特征在于，所述试剂包括用于检测EGFR基因拷贝数的荧光探针RP11-339F13和用于检测7号染色体倍性的荧光探针RP11-144H20；其中，RP11-339F13为覆盖EGFR基因全长的BAC克隆，用罗丹明荧光素标记，其末端序列如下：(1)RP11-339F13正向序列(Forwardsequence)：cagagacaggagcggaggcttctcctggtgaccactctgcttaaaaacttcatcagatccgtagtttcagagcccccctgaaccccatcccttacctctaccagttgcaggtgggtctctggggtggggctgccctccccaccagcaccccaagggctaaaaggttgaggggagaacaccatcatttgtacagggggatcctggaagatgaggcctgagaaagccctgcggggcccctcaccttctccctagctgtggccaagagtgtctggccttgcctgcctcaggaccagcccaaagtggaggtgagaggtgagccccagcccccaggggaagggtgatggtggtcttggtctcagcatggttctggtagaggtgggttattttgaagatgatgaaccttaagcctctttctgatcttgctttaaataaatacttctgaacaacagcaacaacagaatagtgttgataggaaagccctccactccaccagaaccacgcggccttctcgtcctcccctcctccacttccttcctaagtcactgctccatgagctcttccacaggagatttacaaaatagaacacaaacaatccagttcccgcctctcactctgaactcctcccaagactcgtggggtgcggcagcccctgggaacacccagcccttcaaggtcaaacacagcccccgcccctcactctggggtaccctgccagaataagccccgacagccatgtggagcagagccttc(2)RP11-339F13反向序列：ccgcccggccagccgccccgtccgggagggaggtgggggggtccaaggtaaggaaggccctagagtctaggaagagactggccagtgcattaaccattgtagtgtaatggatttgatgttactttatgctcacaagttggacacacacacagggacccagatcaaattggaggggaaggtcaaagtcagcttcccaaaggaactgaagcttagactgatttttaaaggctgagttaaaataaactgggtaaaaataaagaaggaaagagaaaagcaaatgtaaaatatctgtgcagcatgaagtagcaaaatatattcagagaatgtaagaagctgaaatgagggcctcaactaagcgtgggaatgcagatggtgacatagcaatgctgaggtaggctccccaggaccgaggccggcttgttgcggggagaggggaggggcactctaggatgatctctggcgtaagcagcaggtaacgcggtgccaggacatggctgagttttacataaacatttgttcaagcaaatatgggatcattcactgaaacggagaattcaaagtgaggtctagatttggtggcaagatacaactgattcgaggttaggaaaggagagttggaggaacaggaggaagcggcctctcatctgcaaaccctgagttcctggaacacttgtttgttagggagggaagtgtggcccagccacaatcctttgaacatcttcctcttccatcctctgggttctgcatttc；RP11-144H20为7号染色体着丝粒特异的BAC克隆，用异硫氰酸荧光素标记，其末端序列如下：(3)RP11-144H20正向序列：gaattcagcagtttggaaacactctttttgtcaattctgcaagtcgacatttgggaggtcattgagctcaaaggtgaaaaagtgaatatcccataataaaaagtagaagaaatctattgcattgaggcctatggtgaaagaggaaaaatattcagaaaaaatctagaaagaagctttctgagaaactgctttgtgatgtgtgcattcacctaacagagttaatcctttcagtgaaatcagcagtttgcaaacactgttttcatccattctgtaaaaggacatttgctagttcattaacaccaatggcaaaaaagcaaatatcactggagaaaaactagaaggaatctatctgagaaaccactttttgatgtgtgcattcacctcgcagagttaaacttttattttcatggagcaggttgtaaacactctttttgtagaaactgcaaagggatatttgggagcgcatttaggcttatggtaaaaaggaaatatcttaagttgaaaactagaaacaagctttctgagaaacttctttgtgatgtgcacattcatatcacagagtaaaacctttactttggattcagtagtttggaagccctgttgttgtagaatgtgtgaagggatacttgggagctcattgaggccatccggtaaaaagtgaatatcccaggataaaaactaaaaggaaacaatctgagaaaccactatgtcatgtgtgcattcacctcgcagaattaaaactttcttttc(4)RP11-144H20反向序列：ctgagaattcttccgtctagcattcaatgaagaaatcccgtttccaacgaaggcctcaaacaggtccatatttccacttgcagactttacaaacagtgtgtttccaaactcctctatgaaaagaaaggttaaactctgtgagttgaacgcacacatcacaaagcactttctgagaatgattctgtctggttattatacgaagatatttccttttctgcaattgtcctcaaatcgcttgaaatctccacctgaaaatgccacagcaagagtgtttcaaatctgctctctctaaagcaaggttcaactctgtgagttgaatacacacaacacaaaaaagttcctgagaactcttcttagtctagcatgaaaggaagaaaccccgtttgcaacgaagccctcaaagaggtccaaatatccacttgcagacataacaagcagagtgtttctaaactgctctaagaaaagaaaggttaaactctgtgagttgaaggcacacatcacaaagtagtttctgagaatgattctgtctagtttttatttgaagatatttccttttctactgttggcatcaaatcgcttgaaatctccacttgcaaattccacaaaaagagtgtttcaaatctgctctgtgtaaagggacgttccactctgtgagttgaatacacacagcacaaagaagttactgagaattcttctgtctagcatgaaatgaagaaatcccgtttccaacgaaggcctcaatgcggtcca。